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NucleoCounterTM & NucleoCounter YC-100:

Automated Cell Counting Devices for Mammalian Cells & Yeast

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Do You Hate Your Hemacytometer?

Accurate counts in under a minute!

The NucleoCounter® is a unique automated cell counting device for mammalian cultures. The Nucleocounter YC-100 counts yeast. Both are fast, accurate, easy to use, and maintenance- and calibration-free!

Precision Counts, Made Easy!

The NucleoCounter systems use the same basic principles to determine cell concentration as used in conventional methodology involving a hemacytometer and microscope. However, the compact NucleoCounter system adds the precision and reliability of fluorescence microscopy technology. The NucleoCounter automates this time-consuming task, eliminating manual evaluation, and errors caused by operator subjectivity.

The NucleoCounter system uses a disposable sampling device, the NucleoCassette™. This small, plastic cartridge is internally pre-coated with a fluorescent dye, propidium iodide (PI), which stains the cells' nuclei. Because the NucleoCounter detects signals from the stained nuclei, and not the cells, the system operates without regard to cell size or morphology. It is therefore capable of easily counting a variety of culture types, including CHO, hybridomas and adipocytes, without requiring calibration. Aggregate cultures pose no problem, as there is no need for the cell's outer membrane to be intact.

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Disposable NucleoCassette (described at left)

The NucleoCounter system consists of:

  • A NucleoCounter with highly-advanced fluorescence microscope, charged-coupled device (CCD) camera, and hardware-based image analysis
  • Disposable NucleoCassettes with piston (A) to aspirate a pre-defined volume of your sample into the cassette; (B) nuclei-staining dye; and (C) window where sample will be read by the NucleoCounter
  • Reagents for cell lysis and stabilization
  • Optional NucleoView™ PC software for documentation, image viewing and data processing

Simple As 1-2-3 - It's As Easy As That!

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1.

Pre-treat your sample with reagent(s). The lysis and stabilization buffers dissolve cell aggregates and lyse cell membranes.

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2.

Load the NucleoCassette with your pre-treated sample, place in NucleoCounter and press Run. This will produce a total cell count in about 30 seconds.

To determine cell viability, simply run a NucleoCassette with untreated sample. This will count non-viable cells. Cell viability can be determined from these two measurements.

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3.

Or, let NucleoView PC software automatically compensate for dilution and calculate cell viability. This advanced software also allows you to view the sample image, and record data to your PC for report writing.

Clean-Up Is Simple, Too!

After use, the NucleoCassette can be disposed of as biological waste, with the PI dye safely enclosed. Unlike flow systems and hemacytometers, the system requires no cleaning. The NucleoCounter is designed to be maintenance and calibration free. For customers working in a GLP or GMP environment, a new Quality Control Test Kit is available to verify system performance. Ask your rep for details.

Customer Testimonials

"The NucleoCounter is a sleek and convenient alternative to the subjective method of the hemacytometer. The ease of use and lack of cleaning or maintenance makes cell biology considerably easier and more efficient. I have used the competition's particle counters and have found the cleaning and maintenance almost as time consuming as counting by hand. Another advantage of the PI based counting system over the various particle counters is the inability of the trypan-blue based systems to differentiate cells from air bubbles and accurately count aggregated or agglutinated cell clusters. I am more than pleased with my company's purchase of the NucleoCounter and have recommended it to my colleagues in the academic community."
- Corey W. Pine, Research Associate, Amicus Therapeutics, Inc.

"The Nucleocounter has helped our research progress at an amazing rate. Without this affordable and accurate machine, the past few months of research would have taken years. After comparing it to the hemacytometer, Coulter Counter Z1, and Beckman-Coulter ViCell, the Nucleocounter proved to be just as accurate as the much more expensive Vi-Cell. Another plus to Nucleocounter is the sample volume size. With only 75 uL of sample needed, this machine has allowed us to count when only very few cells are available."
- Rachelle Kosoff, Research Assistant, Wistar Institute

"The NucleoCounter is a convenient, compact, reproducible and fast cell analysis system with easily navigable software. Much preferred over trypan blue exclusion or impedance measurement counting."
- Kevin Huff, Biologist, Merck

Availability

This product is available exclusively from NBS in the US, Canada and Mexico.

For further information, see the downloadable PDFs at left, or click here to Request Information.

* NucleoCounter®, NucleoCassette™, and NucleoView™ are trademarks of ChemoMetec A/S, Denmark.

 







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